Fascination About attune flow cytometer

Fascination About attune flow cytometer

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Details-enriched characterization of cell populations. Morphological data from pictures can add to your richness of flow cytometry knowledge. Such as, the determine demonstrates an in any other case traditional apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Every populace to expose morphologically distinct features. These insights couldn't have already been received from multiplex staining on your own.

As samples are acquired within the Attune CytPix Flow Cytometer, the large-speed brightfield digicam captures and merchants pictures of detected activities at a price as many as 6,000 photographs for every next, with regards to the flow charge and picture measurement. For greater flexibility, Attune Cytometric Computer software helps you to alter the graphic capture frequency as necessary and select a selected gate for picture capture, if required.

Combine acoustic concentrating technology with high-parameter Assessment for versatile spectral unmixing and conventional workflows.

Find out new insights into your experimental details by visualizing populations making use of merged fluorescence and morphology details. Our computer software interface wizards enable downsampling, concatenation (merged information) and dimensionality tools that happen to be developed into the computer software for an intuitive, streamlined data Examination experience.

"A picture is not fairly value a thousand dots, but it really seriously really helps to see photographs. It is excellent to see photos that correlate with staining. For instance, AnnexinV-positive cells which can be morphologically distinct than healthful cells, or CD14+ monocytes which have been larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.

Here, an experienced user has gated singlets confidently. Immediately after assessing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate includes a lot more than four% aggregates.

Optimizing gating system. Even strong handbook singlet gating is mistake-susceptible and stays a subjective conclusion point in Nearly all flow cytometry assays. Imaging may be used to confirm and regulate gates to incorporate only solitary cells of interest.

Reliable image good quality even at significant throughput Acoustic concentrating plus a significant-pace camera Mix to impression these T cells continually at very low or superior flow charges. Simply adjust concentration and camera options to meet experimental necessities.

Early signs of this contamination had in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also allows you to use morphological attributes to find out interesting subpopulations that will not be obvious from flow cytometry info on your own.

Perhaps most significantly, these occasions contain cells of Plainly different phenotypes which may lead to incorrect conclusions concerning click here double constructive situations (especially in scarce populations).

AI-pushed automated graphic Examination While using the Attune Cytometric Application interprets function characteristics into unique morphology parameters which might be coupled with common fluorescence and scatter parameters to reveal extremely reproducible populace changes and one of a kind subsets.

Application Take note: Automated impression Investigation cuts down person-to-person variability in flow cytometry gating methods

Constructed-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate envisioned inhabitants resolution in lysed entire human blood. Person can check out occasions from Just about every cluster for Visible confirmation of cell phenotypes.

No clean/no lyse leukocyte Examination with imaging. Cells were obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Pro UX/UI enables customers to coach AI products for expanded applications. (A) The “Train Design” element supplies annotation applications and serious-time model schooling accuracy.

Gating on these prolonged parameters enables you to swiftly and precisely identify populations of curiosity and make sure gating tactic with little or no manual critique. The Attune software package offers use of point out-of-the-art device Understanding Examination resources inside an expertly developed person interface, which makes it exceptionally simple to use.

The Attune Xenith cytometer is often a significant-parameter spectral analyzer, furnishing both of those spectral unmixing and conventional flow capabilities for extensive and multipurpose mobile Evaluation.

Agilent Value Assure guarantees you at the least ten years of instrument use with the day you buy. Or else, We're going to credit history you While using the residual worth of the procedure towards an upgraded product.

In this article, we display that by gating techniques making use of picture-based quantitative parameters can distinguish interacting cells from coincident occasions to much more accurately review interacting cells.